• Драгана Стаменов, MSc. Cтипендиста Министарства просвете и науке
  • Мирјана Јарак, PhD Пољопривредни факултет, Нови Sad
  • Симонида Ђурић, PhD Пољопривредни факултет, Нови Sad
  • Тимеа Хајнал-Јафари, PhD Пољопривредни факултет, Нови Sad


The aim of the investigation was to determine the fertility of acidic soil (pH 5.4) according to the number of microorganisms and their enzymatic activity, as well as to enhance productive properties of this soil using microbial inoculants and calcium carbonate. One half of soil samples were untreated and the investigation focused on examining the number of systematic and physiological groups of microorganisms, dehydrogenase, protease and urease activity. In the other half of samples calcium carbonate (5t/ha) and microbial inoculants were added. The soil was sown with red clover and the number of microorganisms, the length and the dry matter mass were determined 20 and 40 days later. The untreated soil samples were poor in the investigated groups of microorganisms whereas azotobacter was not registered at all. Enzymatic activity was low. As for the soil samples which were treated with calcium carbonate and microbial inoculants, an increase in the total number of bacteria, number of actinomycetes and azotobacter was observed. The greatest length and the dry matter mass of the plant were observed in the variants with rhizobia inoculation, with and without liming.


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1. Aciego, J.C., Brookes, P.C. (2008): Substrate availability and pH as factors controling microbial biomass, activity and community structure in an arable soil, Soil Biology and Biochemistry 51, pp.569-657
2. Alexander, M. ( 1980 ) : Effects of acidity on microorganisms and microbial processes in soil. In: Hutchinson, T.C., Havas, M. (Eds.), Effect of Acid precipitation on Terrestrial Ecosystems. Plenum Press, New York, pp.363- 374.
3. Aquilanti L., Favilli F., Clementi F. (2004): Comparasion of different strategies for isolation and preliminary identifcation of Azotobacter from soil samples, Soil Biology and Biochemistry, Vol.36, Issue 9, pp.1475-1483.
4. Baath, E., Anderson, T.H. ( 2003): Comparison of soil fungal/bacterial ratios in a pH gradient using phisiological and PLFA-based tehniques, Soil Biology and Biochemistry 35, pp. 955-963.
5. Bailey, V.L.,Smith, J.L., Bolton, H. (2002): Ffungal to bacterial ratio in soils investigated for enhanced C sequestration, Soil Biology and Biochemistry 34, pp. 997-1007.
6. Bo L., Gumpertz, M.L., Shuijin, H., Ristaino, J.B. (2007): Long-term effects of organic and synthetic soil fertility amendments on soil microbial communities and the development of southern blight. Soil Biology and Biochemistry 39, pp.2302-2316.
7. Egamberdiyeva, D. (2007): The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils, applied soil ecology, 36, pp.184-189.
8. Hayano, K. (1986) : Cellulose complex in tomato feld soil. Induction, localization and some properties. Soil Biologogy and Biochemistry 18, pp. 215-219.
9. Kološenko, O.T.(1981): Azotofksirajušćije bakteriji- Fiziologija razvitijaBGU, sta.111.
10. Ladd, J.N. (1972): Properties of proteolytic enzimes extracted from soil. Soil Biology and Biochemistry 4, pp.227-237.
11. Jarak, M., Hajnal, T. (2006): Ukupan broj mikroorganizama, broj gljiva I azotobaktera u sabijenom i rastresitom zemljištu. Traktori i pogonske mašine 11, 5, 37-40.
12. Lenhard, G. (1956): The dehydrogenase activity in soil as a measure of activity of soil microorganisms, Z. Pflanzenernaehr. Dueng. Bodenkd., 73, 1-11.
13. Limner, C., Harold L. Drake (1996): Non-symbiotic N2 – fxation in acidic and pH- neutral forest soils: Aerobic and anaerobic differentials, Soil Biology and Biochemistry, Vol.28, Issue 2, pp177-183.
14. Loll, M.J., Bollog, J.M.V. (1983): Protein Transformation in Soil Adv.Agr.36, 351-382.
15. Marschner, P., Yang, C.H., Lieberei, R., Crowley, D.E. (2001): Soil and plant specifc effect on bacterial community composition in the rhizosfere, Soil biology and Biochemistry 33, pp.1437-1445.
16. Melero,S.,Madejon, E., Ruiz, J.C., Herencia,J.F.( 2007): Chemical and biologycal properties of a clay soil under dryland agriculturale system as affected by organic fertilization, Europ.J.Agronomy 26, pp.327-334.
17. Nannipieri,P.,Ascher,J.,Ceccherini,M.T.,Landi,L.,Pietramellara,G.,Renella, G. (2003): Microbial diversity and soil functions, Eur.J.Soil Sci. 54, pp.665-670.
18. Page, L.A. (1982): Methods of soil analysis, Part 2,Agronomy, Madison, Wisconsin USA
19. Rodriguez-Echeverria, S., Perez-Fernandez, M.A. (2004): Potential use of Iberian shrubby legumes and rhiyobia inoculation in revegetation projekts under acidic soil conditions, Applied Soil Ecology, 29, pp.203-208.
20. Romeiko,I.N. (1969): Proteolitičskaja aktivnost dernovo podzolistij počvi pri raznih sposobah paški. Počvovedenije, Vol.10, 87-90.
21. Roscoe, R., Vasconcellos, C.A., Furtini neto, A.E., Guedes, A.A., fernandes, L.A. (2000): Urease activity and its relation to soil organic matter, microbial biomass nitrogen and urea-nitrogen assimilation by maize in a Brazilian Oxisol under no.tillage and tillage systema, Biology and Fertility Soils 32, pp.52-59.
22. Stres, B., Bonete, J.M., Martinez-Espinosa, R., Mahne, I., Bothe, H. (2007): Organisms of the Nitrogen Cycle under extreme conditions: low temperature, salinity, pH value and water stress, Soil Biology and Biochemistry, Vol: 33, Issues 7-8, pp.853-866.
23. Ste-Marie, C. And Pare, D. (1999): Soil, pH and N availability effects on net nitrifcation in the forest floors of a range of boreal forest standsSoil Biology and Biochemistry, Vol: 31, Issue 11, pp.1579-1589.
24. Tabatabai, M.A., Bremner, J.M. (1972) Assay of urease activity in soil. Soil Biology and Biochemistry 4., pp.479-487.
25. Thalmann,A.(1968):Zur Methodikde Bestimmung der dehydrogenase aktivitat im Boden mittels Triphenytetrazoliumchlorid (TTC). Landwirsch. Forsch., 21,pp. 249-257.
26. Trevors,J.T. (1984): Effect of substrate concentration, inorganic nitrogen, O2 concentration, temperatuer and pH on dehydrogenase activity in soil, Pl.Soil.77, pp.285-293.
27. Trolldenier, G. (1996): Plate Count Technique. In Methods in Soil Biology, Springer-Verlag Berlin Heildebergpp.20-26
28. Ulrich, A., Wirth, S. (1999): Phylogenic diversity and population densities of culturable cellulolytic soil bacteri across an agricultural encatchment, Microbial Ecology, 37, pp. 238-247.
29. Van Overbeek, L.S., Van Vee, J.A., Van Elsas, J.D. (1997): Induced reporter gene activity, enhanced stress resistance, and competitive ability of a genetically modifed Pseudomonas fluorescens strain released intoa feld plot planted with wheat, App.environ.Microbiol., 63, pp.1965-1973.
30. Wardle, D.A. ( 1992 ): A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil, Biological Reviews of the Cambridge philosophical society, 67, pp. 321-358.
31. Wittmann, K., Suominen K., and Salkinoja-Salonen, M.S.(2003b): Evaluation of ecological disturbance and intrinsic bioremediation potential of pulp mill contaminated lake sediment usin key enzymes as probes. Environmental Pollution 107, pp.255-261.
32. Whitelow M.A. (1999) : Growth Promotion of Plants inoculated with Phosphate- Solubilizing Fungy, Advances in Agronomy, Vol.69, pp.99-181.
How to Cite
СТАМЕНОВ, Драгана et al. THE INCREASE OF MICROBIOLOGICAL ACTIVITY OF ACIDIC SOIL BY MEANS OF INOCULATION AND LIMING. Economics of Agriculture, [S.l.], v. 58, n. 1 Book 1, dec. 2011. ISSN 2334-8453. Available at: <>. Date accessed: 03 mar. 2021.